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Introduction of shRNAs, miRNAs or anti-microRNAs into primary human hepatocytes with lentivirus

Uploader: Maria Thomas

Contributor: Maria Thomas

Other contributors: Not specified

Version: 1

Projects: A3.4: Linking signalling to metabolic functions, B5: Cell-cell communication influences detoxifying functions in hepatocytes

Scales: Not specified

Here we used VSV-G-pseudotyped, EGFP-expressing lentiviral vectors to develop an efficient gene transfer protocol to modify gene expression in primary human hepatocytes (by RNAi). The protocol comprises the production of recombinant viruses as well as the steps for efficient delivery of short-hairpin RNA (shRNAs), microRNAs or anti-microRNAs to human hepatocytes. On average infection efficiencies of over 95% are achieved at relatively low multiplicity of infection (MOI), which effectively reduces
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Created: 4th Apr 2011 at 17:06 Last updated: 21st Jan 2013 at 12:16

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