Mesenchymal stem cells are extracted from the tissue by collagenase digestion and enriched by their capacity to grow on plastic surfaces. Enriched cells display distinct mesenchymal surface markers and are capable of multiple lineage differentiation. In the presence of specific growth conditions, the cells adopt functional features of differentiated hepatocytes. After orthotopic transplantation, differentiated human stem cells engraft in the host liver parenchyma of immunocompromized mice. This protocol describes the in vitro differentiation of stem cells from human bone marrow and their transplantation into livers of immunodeficient mice.
24
hidden item
Projects: C6: Organization and function of the sinusoidal system and the liver lob..., Showcase Steatosis
Not available
Experimentalists: Not specified
Contributors Related items
